Two-photon excitation (TPE) microscopy enables imaging of living tissues up to a depth of one millimeter, which is 6 to 10-fold deeper than with confocal microscopy. Like confocal microscopy, TPE ...

a multi-photon (or 2-photon) microscope uses a pulsed infrared laser to stimulate fluorescence excitation only with the diffraction limited spot of the objective lens, thus creating an inherent ...