(H) Increases in stemness marker genes KLF4 and Sox2 were verified by western blot. (I) Akt1 phosphorylation was increased upon PirB depletion as shown by western blot. (J) Working model for PirB ...

which is then phosphorylated by AKT1. This phosphorylation is inhibitory and prevents GSK3β from phosphorylating SRPK1. As a result, SRPK1 remains cytosolic, nuclear phosphorylation of SRSF1 is ...