The yield of final products was greatly improved by using a high concentration of megaprimers and asymmetric PCR. A PCR–based approach was developed that provides a powerful tool for engineering ...

The problem with asymmetric PCR, which requires dilution of one of the two primers to make it a limiting primer, is that the decrease in the melting temperature of that limiting primer upon ...

The protocol for the enhanced FBI-seq unexpectedly mirrors a simplified Thermal Asymmetric Interlaced (TAIL)-PCR, a technique widely employed for isolating flanking sequences. However, the improved ...