The PamChips® are blocked in 2% Bovine Serum Albumin (BSA). Following protein concentration determination (by BCA assay), 5-10 μg of protein for the PTK chip (~1 μg for the STK) will be loaded per ...
β-Parvalbumin is a calcium-binding protein prevalent ... impact the assay’s performance. This success was attributed to the careful optimization of blocking agents like BSA, with the optimal ...