The precise insertion of DNA sequences using the homology-directed repair (HDR) pathway is one potential use of the CRISPR/Cas genome ... found to be 80% higher. GFP KI at RAB11A Locus in HEK293 ...

This image shows a schematic diagram of the highly efficient CRISPR/Cas system, which leads to super efficient targeted insertion (knock-in) of a long donor insert into mouse genome.

We provide transgene expression in mammalian cell lines using lentiviral and retroviral transduction and gene knockout and knockin using CRISPR/Cas9 technologies. 1. STABLE CELL LINE GENERATION ...

CRISPR-based homology-directed repair (HDR ... achieving precise changes via HDR remains a challenge, particularly for large knock-ins. These insertions can be generated via HDR using enzymatically ...