The lysate is filtered through a 0.2-μm filter, and the cleared cell lysate is loaded on to the spin column at an optimal flow ... Purification of His-tagged wild-type and mutant recombinant ...

The use of nickel in immobilized metal affinity chromatography, as during isolation of His-tagged proteins, can hinder the purification of recombinant ... flow rate by increasing the filtration ...

Technology Networks Ltd. needs the contact information you provide to us to contact you about our products and services. You may unsubscribe from these communications ...