In principle, cloning PCR products necessitates some fancy footwork on the part of the researcher. But in practice with the help of a battery of kits, all you need to know is the nature of the ...

In step 2, the gene fragment is integrated into a circular piece of DNA, called a plasmid, which is the main delivery vehicle into the cell and contains an antibiotic resistance gene for plasmid ...

Figure 2 shows the configuration of a sample template DNA oligonucleotide derived from the RNA oligonucleotide design. Synthesize or order the template oligonucleotide designed in steps 1 and 2 ...