During these thermal cycles, DNA primers bind to the target DNA sequence, enabling DNA polymerases to assemble copies of the target sequence in large quantities. PCR makes it possible to produce ...

At the Nevada Genomics Center we offer DNA sequencing using dye-terminator Sanger sequencing with analysis on an Applied Biosystems SeqStudio Genetic Analyzer. A Sanger sequencing reaction is run with ...

During circular DNA replication, the excised primer is readily replaced by nucleotides, leaving no gap in the newly synthesized DNA. In contrast, in linear DNA replication, there is always a small ...

“Niall’s idea was to use recombinase proteins like RecA to target DNA primers to specific sites,” said Piepenburg. “This idea evolved into the notion that if you have two primers that are interacting ...

“But the idea that you could amplify a specific targeted DNA sequence using an oligonucleotide primer and DNA polymerase was not at all obvious.” Erlich led the team of scientists at the Cetus ...

Original DNA template - DNA is a double helix made of two complementary strands. Each strand can be used as a template to create a new DNA molecule. Free DNA nucleotides – needed to form the new ...