To avoid these limitations, multiphoton LSM uses a nonlinear fluorophore excitation process in combination with a non-descanned detection concept to greatly increase the penetration depth.

Applications include many types of multiphoton excitation microscopy ... bright imaging of UV-absorbing fluorochromes, such as DAPI and Hoechst, as well as those requiring longer excitation ...

Experimental setup for the multimodal nonlinear optical microscopy of multiphoton excitation fluorescence (MPEF) and second harmonic generation (SHG) imaging. A femtosecond pulse from a tunable ...

Optimized for reaching greater imaging depths, the system also features simultaneous dual-wavelength excitation (up to 1300 nm). Visible or multiphoton laser light stimulation and the ability to ...