Nature10 年
Detecting protein-protein interactions with GFP-fragment reassembly
To prepare the cloning vectors, transform separately pET11a-link-NGFP and pMRBAD-link-CGFP into an E. coli strain that gives ... Purify the vector products by electrophoresis through 0.8–1% ...
scmp.com27 天
‘Very important step’: Chinese scientists turn E coli into a photosynthetic life form
It is a common gut bacteria that never normally sees the light of day, but now Chinese scientists have engineered E coli so that it can harness sunlight like a plant. Incorporating a custom ...