Grow the MEF cultures to confluency, collect the cells and freeze (one vial/flask) as described in Steps 16–20 These are passage-zero MEFs. Split each flask (from Step 3) 1:5 to generate 25 ...

The roles of epithelial-mesenchymal transition (EMT) and immune cells are crucial in tumor metastasis. This study aimed to investigate the interaction between immune cells and EMT of tumor cells in ...