In cells without FUT8, the mucous layer is thinner and easily removed by washing (left). Cells with high levels of FUT8, as observed in Ulcerative Colitis patients, produce a more permeable mucus ...

Methods: An anti-BDCA2 antibody was generated from mouse hybridoma and humanized by CDR grafting. The afucosylated antibody was then produced using fucosyltransferase inhibitor or a FUT8 knock-out ...