Most FRET-based applications rely on hetero-FRET ... As this is a cellular process that needs to be recorded in a cellular background, common biochemical assays fail to record changes in packaging.
The specific signal generated by fluorescence resonance energy transfer (FRET ... In a direct, homogeneous assay compatible with HTS conditions, cells expressing the target of interest are ...
These provide the highest flexibility while delivering filter-like performance for fluorescence and luminescence assays including FRET and BRET. There is no compromise when it comes to sensitivity ...