and assemble the gel cassettes into the second-dimension electrophoresis apparatus. Fill the electrode chambers with the appropriate electrode buffer. 10| Push the IEF gels from the glass tubes ...

Run the gel in the same buffer solution as used for preparing ... range of DNAs to be resolved (as described below) and continue electrophoresis for 12–18 h. TAFE settings for separating DNAs ...

Biotium also offers accessory products like 1X TAE or TBE Buffer Powder Packets to further streamline gel electrophoresis workflows. For best results, Biotium recommends imaging with the Gel-Bright™ ...