Figure 1: Retroviral backbone of pSHAG-MAGIC2. To generate the hairpin primer, select a 'sense' sequence(s) of 22 nucleotides (nt) in length from the coding sequence of the gene of interest for ...

with the primer at the 3' end and the probe contained within a hairpin-loop structure at the 5' end. Scorpions Primers utilize a uni-molecular mechanism that acts faster in solution for ...