Figure 1: Retroviral backbone of pSHAG-MAGIC2. To generate the hairpin primer, select a 'sense' sequence(s) of 22 nucleotides (nt) in length from the coding sequence of the gene of interest for ...

Another chemistry that uses hairpin structure is that of Scorpions, first developed by David Whitcombe at DxS 4. Unlike beacons, Scorpions are PCR primer and probe in one. They consist of a primer ...

with the primer at the 3' end and the probe contained within a hairpin-loop structure at the 5' end. Scorpions Primers utilize a uni-molecular mechanism that acts faster in solution for ...