HindIII, for example, is an H. influenzae restriction enzyme that recognizes the sequence 5'AAGCTT-3' (upper strand)/3'TTCGAA-5' (lower strand) and cleaves between the two A's on both strands.
Prepare the restriction enzyme digests. You will perform two single digests using enzymes EcoRI and HindIII and one double digest using both enzymes. You will also be given some plasmid prepared by ...