The isopentane will start to become opaque as it nears freezing. Take the isopentane out of the liquid nitrogen and freeze the specimen as described above. Chill the isopentane again as necessary for ...
Transfer ∼100 mg of the frozen tissue to a mortar containing liquid nitrogen and pulverize the tissue using a pestle. Keep the tissue frozen by the addition of liquid nitrogen. iii Transfer the ...
but it could establish Chart’s liability for the loss of reproductive tissue belonging to hundreds of other people. The tank contained 3,500 frozen eggs and embryos. A mechanical engineer previously ...