Take the isopentane out of the liquid nitrogen and freeze the specimen as described above. Chill the isopentane again as necessary for subsequent tissues. This method has the advantage of very rapid ...

Medically reviewed by Archana Sharma, DO Cryosurgery is a localized treatment that uses extreme cold from liquid nitrogen or ...

Transfer ∼100 mg of the frozen tissue to a mortar containing liquid nitrogen and pulverize the tissue using a pestle. Keep the tissue frozen by the addition of liquid nitrogen. iii Transfer the ...