Positively charged nylon membrane, alkaline transfer. Stain with methylene blue (see below). Uncharged or positively charged nylon, neutral transfer. Fix by UV irradiation and then stain with ...
4| After electrophoresis is complete, stain the gel with ethidium bromide ... For transfer to charged nylon membranes: (i) Soak the gel for 15 min at 15–25 °C in several volumes of alkaline ...
Most bacteria can be divided into two groups based on the composition of their cell wall: Gram+ and Gram- This is The Most Important staining technique in Bacteriology. Gram-positive cell walls have a ...
Immunofluorescence microscopy disclosed linear staining of the glomerular capillary basement membrane for IgG, without concomitant staining of the tubular basement membrane. By contrast ...