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Prior to the development of PCR in the 1980s, the primary method for producing many copies of a gene was a relatively time-consuming process known as DNA cloning. This technique involved insertion ...

7 This allows the amplification of long DNA molecules which cannot be amplified by regular PCR, especially genomic DNA. PCR for cloning PCR for cloning amplifies DNA fragments for ligation into a ...

The PCR products of today are more accurate replicates of their templates, and there is a kit for nearly every possible way of cloning a DNA fragment (from transcribed RNA or another source ...

PCR-machines are used for replication of DNA, detecting DNA sequences, carrying out DNA fingerprinting, forensic analysis, and molecular cloning, diagnosing genetic diseases, and detecting ...

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The global gene synthesis market size was valued at USD 2.20 billion in 2024 and is projected to reach from USD 2.55 billion in 2025 to USD 9.54 billion by 2033, growing at a CAGR of 15.8% during the ...

Applications of PCR include the cloning of known and novel genomic DNA and cDNA sequences, DNA sequencing, construction of mutant or chimeric DNAs, and quantification of mRNA and DNA. PCR is also used ...

In-Fusion HD Cloning Plus是一款简便高效的克隆试剂盒 ... 已采用Clontech In-Fusion 克隆系统构建了pOPIN vector 重组载体库 。OPPF科学家以703个PCR片段为目的片段成功构建了661重组载体，总体克隆效率可 ...