Real-time PCR combines the amplification of a DNA sequence with the detection of the amplified products during each reaction cycle—in other words, in real time. In comparison to conventional PCR ...
In recent years, quantitative real-time PCR tests have been extensively developed in clinical microbiology laboratories for routine diagnosis of infectious diseases, particularly bacterial diseases.
Thus, the overall efficiency of RT– and real-time PCR in detecting the housekeeping gene in a limited number of picked cells was 82% (243/297 samples). Negative samples may be mostly caused by ...
As the coronavirus that causes the COVID-19 disease spreads across the world, the IAEA, in partnership with the Food and Agriculture Organization of the United Nations (FAO), is offering its support ...
Download this application note to learn how the microbial detection capabilities of real-time PCR-based bacterial and fungal kits compare to those of culture-based sterility testing. You can withdraw ...
Extension: Nucleotides are added to the primers, which serve as extension points to continue new strand synthesis. Endpoint analysis: Scientists analyze PCR products typically using agarose gel ...