Scientists commonly do this using gel electrophoresis analysis, which can determine plasmid length and conformation based on how DNA migrates within a gel. The Agilent Fragment Analyzer system offers ...

Figure 3: 1% agarose gel tracing the large-scale purification of pBGS19Luxwt plasmid DNA. Anion-exchange chromatography is commonly used for final purification of plasmid DNA 13. It was found that ...

Gel electrophoresis is a laboratory method used to separate mixtures of DNA, RNA, or proteins according to molecular size. In gel electrophoresis, the molecules to be separated are pushed by an ...

During this week you will carry out restriction digestion of the genomic clone, in order to cut the insert (=the gene) from the plasmid DNA (=vector). Following separation of insert and vector by ...