Two-dimensional polyacrylamide gel electrophoresis (PAGE) is used for separation of complex protein mixtures by the independent parameters of isoelectric point and molecular weight. Isoelectric ...

MS, an experimental protocol combining high-resolution protein fractionation using SDS-polyacrylamide gel electrophoresis (SDS-PAGE) with the latest mass spectrometry technology to enable highly ...

We find purification of the oligonucleotides by polyacrylamide gel electrophoresis to be costly and unnecessary. Set up the amplification reaction (one for each short hairpin sequence to be tested).

Expression of proteins in the renal cortex was determined by colloidal Coomassie blue staining after two-dimensional polyacrylamide gel electrophoresis. Differential protein spots were excised and ...