This protocol was adapted from Northern blotting: Transfer of denatured RNA to membranes. In Molecular Cloning: A Laboratory Manual (eds. Sambrook, J. & Russell, D.W.) 7.35–7.41 (Cold Spring ...

The main difference between Southern, northern, and western blot techniques is the target ... The main steps in a western blot protocol are as follows. Sample preparation, including protein extraction ...

One advantage of Northern blotting is the ease with which comparative analyses can be carried out, as researchers can run RNA samples representing different developmental time points, tissues, or ...

Many scientists were therefore relieved by the subsequent development of modified northern protocols using blotting conditions that made possible the rapid and efficient transfer of RNAs onto ...