Precipitate the DNA with ethanol in the presence of 0.3 M sodium acetate (pH 5.2) and dissolve in 90 μl of Tris/EDTA (TE) buffer (pH 7.6). 10| To the DNA, add 10 μl 10× T4 polynucleotide kinase ...
Expert Rev Ophthalmol. 2009;4(1):59-64. Edetate disodium (EDTA) is a chelating agent used in a variety of nonophthalmic products, including hair conditioner, facial cleansers, aftershaves and ...
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