We select 1 slide in every 50 (in printing order) for total protein staining and use that to perform total protein normalization across all the samples. The normalization method is described below: A ...

One significant advancement is the introduction of the Stain-Free total-protein normalization method, which has been shown to improve the reproducibility of Western blot data. This method ...

Protein normalization is a critical step in obtaining reliable and reproducible quantitative western blot data. Total protein normalization is considered the gold standard for quantitative western ...