Two-photon excitation (TPE) microscopy enables imaging of living tissues up to a depth of one millimeter, which is 6 to 10-fold deeper than with confocal microscopy. Like confocal microscopy, TPE ...

This important study conducted experiments to quantify how changes in blood flow results in apparent fluorescence changes when imaging neural activity sensors using two-photon microscopy. While the ...

To extend this assay to cells in physiological preparations, we integrated two-photon uncaging and imaging (infrared-LAMP) to monitor intercellular dye diffusion in three dimensions (see ...

His group scans these neurons using two-photon (2P) imaging. However, conventional 2P imaging systems operate only in a 2D plane, but neuronal networks are dispersed in three-dimensional space.