The first step in a western blot is to prepare the protein sample by mixing it with a detergent called sodium dodecyl sulfate, which makes the proteins unfold into linear chains and coats then ...

The applications of western blot are often to the early stages of a study, validating the successful transfection of a cell colony with a non-viral vector, detecting the presence of a specific protein ...

For example, if you’re using western blotting to analyse protein levels, it’s worth knowing that large proteins take longer to travel through gel than small ones, and that the acrylamide ...