According to Balderas, there is an art and a science to developing high-parameter flow cytometry antibody panels. Mixing and matching up to 28 different fluorophores attached to different ...

Charlene earned her MSc and PhD in cell biology from the University of Toronto and currently serves as an assistant science editor for The Scientist's Creative Services team. View Full Profile. Learn ...

Flow cytometry is the current gold standard for identifying cell types within a mixed population. It identifies cells by their unique combinations of markers, which are tagged with antibodies bound to ...

Primary antibody labelling hits the sweet spot With this kit, called FlexAble from Proteintech, primary antibodies can be labelled with one of several fluorophores in about 10 minutes. This not ...

When using DAPI most antibody fluorophores can be used except for Pacific Blue ... This phosphorylation results in the recruitment of DNA damage repair proteins to the site of the DNA damage. Flow ...

The experimental design of a multicolour flow cytometry experiment has a number of issues that should be carefully ... digital compensation and the use of single colour compensation beads for antibody ...

The University's flow cytometry facility is a core facility based in the Royal Hallamshire Hospital and provides a service to all at the University and externally. Flow cytometry is a technique that ...

The Flow Cytometry Shared Core is a user-friendly facility serving CU Boulder and the ... We provide instrument training on our 2 analyzer, assistance with fluorophore selection to best optimize ...