Figure 1: Delivery of synthetic shRNA into Nanog-GFP mouse ES cells. M15 ES cell medium with leukemia inhibitory factor (LIF): DMEM Knockout (Gibco), 15% fetal bovine serum (FBS; StemCell ...

Complete the design of the template oligonucleotide by ... This PCR kit, however, can be used for individual miR-30 shRNA cloning experiments with reasonable success. Analyze an aliquot of the ...