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7、shRNA表达载体的构建 提供shRNA表达克隆订制服务。采用慢病毒系统,以U6启动子驱动克隆的shRNA表达,同时载体上还包括puromycin抗性基因和eGFP荧光报告基因相应表达,以利于miRNA的功能筛选和miRNA表达示踪。
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