Methods: Patients with systemic infection (n=27), active autoimmune inflammatory disease (n=44), vasculitis (n=5), and controls (n=20) were studied for neutrophil CD64 expression using monoclonal ...

These neutrophils were then subjected to scRNA ... This upregulation was validated through flow cytometry and immunohistochemical staining, which confirmed the increased expression of the CCRL2 ...

Neutrophil apoptosis and necrosis were assessed using flow cytometry and morphology and the supernatant was analysed for concentrations of inflammatory mediators. Results: Neutrophil numbers (×10 6 ...

Flow cytometry and confocal microscopy were used to analyze the distribution of LAMP1 in neutrophils under steady state and after activation. Results: Patients with lupus nephritis displayed increased ...

Pulmonary neutrophil accumulation results in release of chemokines ... Approval was from the Lothian Research Ethics Committee (13/SS/0157, 15-HV-013). Flow cytometry data were analysed using FlowJo ...

1 SBP is diagnosed if neutrophil count exceeds 250/mm 3 (or 0.25 × 10 9 at flow cytometry) in ascites. Cell count >500/mm 3 carries greater sensitivity. 2 In our unit flow cytometry is not validated ...

TJ and IC performed experiments and analysed data. CJM, MV and JvdG performed experiments. EW contributed to flow cytometry experimental design and measurements. SSR collected human tissue samples and ...