To determine cell density for subsequent staining, cell counting was performed using Countess II. Afterwards, cells were fixed and stained with FxCycle™ PI/RNase Staining Solution (Invitrogen) for 30 ...

Agilent presents a fully automated, kinetic imaging assay to monitor both cancer cell proliferation and apoptosis in a high-throughput, 384-well format, to deepen insights while boosting productivity.

“Instead of reading cell-based assays ... kinetics, proliferation, calibration and statistical comparisons. Other examples include BD CellQuest, a popular commercial flow cytometry data ...

Cell proliferation is the process that results in an increase of the number of cells, and is defined by the balance between cell divisions and cell loss through cell death or differentiation.

To support physicians in treating cancer patients, oncology laboratories can benefit from adopting end-to-end automation of flow cytometry workflows ... variability in cell population frequencies ...

The Flow Core Currently has a BD FacsVantage equipped with 5 lasers and 8 detectors as ... Users are welcome to run experiments or sorts that comply with the posted guidelines. The FacsVantage can ...

On day 6, cells were harvested for flow cytometry staining and analysis. For the proliferation assay of murine CD8+ T cells, single peptide and repeat peptide stimulated cells were cultured from day 0 ...

They will learn how to design robust FCM experiments and the inclusion of relevant controls for different assays. The students will get insight into labeling procedures and important applications for ...

Water flow plays a surprising role in ... process to siphon off resources to support their proliferation. New branches form through special cells at the leading edges of blood vessels ...

Dronedarone emerged as a potent inhibitor of ESCC cell proliferation. Through a series of in vitro experiments, including cytotoxicity assays, cell proliferation tests, and anchorage-independent ...

We thank Eva Rudolf, Preeti Gupta and Christin Danneil for expert technical assistance as well as the staff at the Animal Facility and the Histology and Flow Cytometry Core Facilities at the ...

of precursors adopting a recognized cell fate. BrdU-labeled cell phenotype was determined by confocal analysis (full z-stack resolution). Only those nuclei clearly associated with the indicated ...