more genomic DNA fragments will be amplified by a primer that contains a single unique base at its 3′ end compared to a primer that contains three unique bases at its 3′ end. Primer design ...
At the Nevada Genomics Center we offer DNA sequencing using dye-terminator Sanger sequencing with analysis on an Applied Biosystems SeqStudio Genetic Analyzer. A Sanger sequencing reaction is run with ...
Annealing: Short DNA fragments bind complementary regions of the template to begin new strand synthesis. Extension: Nucleotides are added to the primers, which serve as extension points to continue ...
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