After washing cells were then labelled intracellularly with anti-B-Catenin-FITC and then stained with 1ug/ml DAPI for cell cycle analysis. All populations of cells were then analyzed for DNA content ...

The authors examine the role of Numb, a Notch inhibitor, in intestinal stem cell self-renewal in Drosophila during homeostasis and regeneration. This is an important study providing evidence of a Numb ...

Hair follicle (yellow arrow) and epidermis were derived from EGFP-labeled PSC. p63: stem cell marker, DAPI: nucleus, KRT14: differentiated keratinocyte marker Scale bars, 20 µm.

After treatment, cells were washed to remove excess probes and stained with DAPI for flow cytometric analysis. Data were processed using FlowJo software to identify live, single-cell populations ...