Live neutrophils were determined as DAPI-negative (>99%) and CD45+CD66b+ (>95% ... The fluorescent DNA-chelating dye Sytox Green (Thermo-Fisher Scientific, USA) was used to stain free DNA for ...

For dividing bacteria with a clear septum, the length was measured until the septum. 2.7 Spinning disk confocal microscopy (SDCM) of biofilms Biofilms formed in the presence or absence of AA were ...

DNA-staining dyes such as propidium iodide (PI) and DAPI commonly exhibit nonspecific binding with RNA due to similar base-pairing behaviors in DNA and RNA, and both molecules exhibit minor grooves ...

In this study, we demonstrate the feasibility of generating virtual stains for hematoxylin and eosin (H&E) and a multiplex immunofluorescence (mIF) immuno-oncology panel (DAPI, PanCK, PD-L1, CD3, and ...

Slides were then washed 3 times with TBS and cover-slipped using mounting media containing DAPI (Thermo Scientific, CAT#P36931). Staining controls included omission of primary or secondary antibodies ...

For cell culture studies, an Alamar Blue assay kit, lactate dehydrogenase (LDH) assay kit, AOPI staining solution, DAPI staining solution, and ActinGreen staining solution were purchased from Thermo ...

The best carpet stain removers can be a lifesaver. Many of us have been there, and witnessed that moment when time slows down and a glass of red wine tumbles and splashes across your light carpet.

Prior to analysis, the DNA samples were stained with 320 nM YOYO-1 (Invitrogen) in 0.5× TBE, supplemented with 2% β-mercaptoethanol (BME, Sigma-Aldrich) to prevent photobleaching, in a final volume of ...

DNA nanorobots can alter artificial cells Date: January 13, 2025 Source: Universitaet Stuttgart Summary: Scientists have succeeded in controlling the structure and function of biological membranes ...

Dalton, GA, Mar. 4--Utilizing the same components that made Shaw's patented* R2x Stain and Soil Repellent System one of the most popular carpet protective treatments in the industry, the company ...