Thermo Fisher Scientific). Chemical reagents used were as follows: 4′,6′-diamidino-2-phenykindole (DAPI) (Cat# 1331762; Bio-Rad Laboratories), Vectashield (Cat# H-1000; Vector Laboratories), PKH26 Red ...
Finally, sections were processed using ProLong Gold Antifade Mountant with DAPI (Thermo Fisher, Waltham, MA, USA) for DAPI nuclear stain and mounting. IF images were captured using a Leica TCS SP8 X ...
Afterwards, the sections were washed in PBST, counterstained for 20 min with 5 ng/ml DAPI (Thermo Fisher Sci., Waltham, MA), and mounted under coverslips with Prolong Glass (Thermo Fisher). For ...
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