Brains of offspring were homogenized in RIPA buffer (20 mM Tris–HCl pH 7.5, 150 mM NaCl, 1 mM EDTA, 1% Triton-X100, 0.5% sodium deoxycholate, 1 mM PMSF, and 10 μg/ml leupeptin), incubated on ice for ...