Understanding bacterial growth mechanisms can potentially help uncover novel drug targets that are crucial for maintaining cellular viability, particularly for bacterial pathogens. In this important ...

Cytosolic and nuclear fractions were prepared from cell lysates and proteins were analyzed by Western blotting for the subcellular expression of HA-miniTurbo, p65-HA-miniTurbo or the endogenous p50 NF ...

Treat cells with or without MG132 (10 µM) for 12 h prior to harvest. The whole-cell lysates were incubated with protein G agarose beads conjugated with anti-Flag antibodies and used for IB with ...

Laboratory of Chemical Biology, Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven 5600 MB, The Netherlands ...

The raw PCR product can then be used as template in a coupled ... Flag sequence (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys), and the test peptides were purified with the M2 anti-Flag monoclonal antibody ...

Instituto de Biología Molecular y Celular de Plantas (Consejo Superior de Investigaciones Científicas − Universitat Politècnica de València), 46022 Valencia, Spain ...

Endogenous HDAC2 was immunoprecipitated along with Flag–DNMT1-a and HA-DMAP1 by anti-HA antibody only in those extracts where HA-DMAP1 was present (Fig. 4c). Likewise, antibodies against ...

Importantly, the cytotoxic activity of neutrophils driven by therapeutic EGFR or HER2 antibodies in vitro was increased by blocking the sialic acid/Siglec interaction, either by reducing tumor cell ...

Development of reliable multiplex assays on xMAP beads for serological or other assays, is not only dependent on proper coupling of capture molecules, it is also dependent on the proper mixing of ...