Even old, degraded DNA yields enough signal to perform the analysis. For maximum signal strength I have optimized the protocol, and several points have to be kept in mind: (1) Fluorescent-labeled ...

The samples may be stored at this point at −20 °C for up to 2 months, or used directly for incorporation of fluorescent dyes using the Megaprime DNA labeling kit (Amersham) if you started with ...

Normally, this requires complex DNA sequencing after imaging to determine ... as the required fluorescent labeling had to be individually attached to each protein. The research group led by ...

3 A labeled probe and target DNA are denatured and mixed to allow complementary DNA sequences to anneal. The fluorescent ...