Most FRET-based applications rely on hetero-FRET ... As this is a cellular process that needs to be recorded in a cellular background, common biochemical assays fail to record changes in packaging.

interacting proteins can be detected with fluorescence-based two-hybrid systems that use split GFP or fusions to AFP pairs that exhibit FRET. Assays that use fluorescence as the read-out have been ...

These provide the highest flexibility while delivering filter-like performance for fluorescence and luminescence assays including FRET and BRET. There is no compromise when it comes to sensitivity ...