The purified proteins were immediately dialyzed into 10 mM HEPES-KOH, pH 7.5, 10 mM KCl, 2 mM TCEP, 10% glycerol, concentrated and either characterized immediately or flash frozen in liquid nitrogen ...

2015). BHK-21 cells transfected with wild-type (WT) or mutant transcribed RNA were trypsinized and washed in PBS three times. The cell pellets were resuspended in hypo-osmotic buffer (10 mM HEPES-NaOH ...

Here we explore peptide inhibitors of Cdc20, a substrate-recognition subunit and activator of the E3 ubiquitin ligase the anaphase promoting complex/cyclosome (APC/C) that is essential in mitosis and ...

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Qatar’s prime minister, Sheikh Mohammed bin Abdulrahman bin Jassim Al Thani, has demanded that Israel “immediately withdraw” its forces from the United Nations-established buffer zone with ...

DAMASCUS, Jan 16 (Reuters) - Syria's de facto leader Ahmed al-Sharaa said on Thursday his country is ready to welcome UN forces into the UN established buffer zone with Israel. "Israel's advance ...

This manuscript describes an important finding of the transcriptional control of a chimeric gene transfer agents (GTA) cluster in Bartonella by a processive anti-termination factor (BrrG). The ...

Department of Applied Chemistry, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama, Kanagawa223-8522, Japan Department of Biosciences and Informatics, Faculty of ...

US President-elect Donald Trump wants members of the NATO military alliance to devote five percent of their national output to defence, a demand that has already been rejected as too high by German ...

MANILA, Philippines — Some parts of the country will experience a rainy Sunday due to the northeast monsoon, or “amihan”, and the easterlies, the Philippine Atmospheric, Geophysical, and ...