Much of what biologists know about the human immune system has been — quite literally — illuminated by flow cytometry. For more than 70 years, this technology has evolved to become one of ...

for macrophages (Mφ) in immunohistochemistry (IHC) and flow cytometry (FACS). Methods: IHC was performed on cryostat sections of rheumatoid arthritis (RA) and osteoarthritis (OA) synovial membranes ...

The cells were counted, and freshly isolated SVCs were stained immediately for flow cytometry analysis. Macrophage surface protein characterization and verification ... Surface proteome screening was ...

In vivo perturbations and single-cell RNA-seq reveal cell-type-specific STAT1-IFNg signaling in regulation of trained immunity in tissue-resident immune cells.

HSF1 transiently accumulates in the nucleus of human monocytes undergoing macrophage differentiation, including M-CSF-treated peripheral blood monocytes and phorbol ester-treated THP1 cells.

This, combined with reduced NK cell cytotoxicity that hampers their ability to kill specific macrophage populations (6), has been shown ... proteome screening to identify novel proteins expressed by ...

Methods Human Huh7 and murine Hepa1–6 HCC cells were treated ... RT-qPCR and western blot were used to identify the As2O3-targeted signals in macrophages, and the signaling was confirmed in the ...

Body Weight,Culture Medium,Dose-dependent Manner,Flow Cytometry,Humoral Immunity,Immune Function,Immune Organs,Immune Response,Immunosuppressed Mice,Innate Immune ...

We found significant monocyte/macrophage infiltration ... were injected with mouse-anti human ß2 glycoprotein I (ß2GPI) monoclonal antibody to induce OAPS. The expressions of CCL2 and CCR2 in decidua ...

Streptococcus gallolyticus (Sg) is a commensal bacterium of the human gut but is also known to be an opportunistic ... Tumor growth and indicators were measured by mouse colonoscopy, and macrophage ...