Briefly, PCR was performed with 2 × KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Inc., United States) under the following conditions: initial denaturation at 95°C for 15 min, followed by 30 cycles ...
A method for genetically barcoding Drosophila is developed and used to tag defined cell populations in vivo for single-cell transcriptomics experiments and to enable multiplexed behavioral analysis.
A new study finds that sharks and rays harbor several harmful bacteria, some resistant to antibiotics, raising concerns over their impact on public health.