Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, Indiana 47907, United States Birck Nanotechnology Centre, Purdue University, West Lafayette, Indiana 47907, ...

or propidium iodide (PI) to a final probe concentrations of 10 μM for NPN or 15 μ M for PI. After incubation at 37 ℃ for 0.5 h, bacterial suspensions were then mixed with different drug combinations ...

Briefly, after initiating the reactions by adding 15 μL of substrate solution, fluorescence was measured with the Multiskan MK3 spectrophotometer at excitation and emission wavelengths of 355 and 460 ...

Cells were harvested and resuspended in binding buffer containing annexin V-FITC and propidium iodide (PI). Samples were incubated for 15 min at room temperature protected from light and analyzed by ...

Department of Chemistry, Sardar Patel University, Vallabh Vidyanagar, Gujarat 388120, India ...

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Solution 5 contains VitaBright-48, which fluoresces when bound to reduced thiols, acridine orange to stain all cells, and propidium iodide to stain dead cells ... The fluorescence intensity was ...

DNA-staining dyes such as propidium iodide (PI) and DAPI commonly exhibit nonspecific binding with RNA due to similar base-pairing behaviors in DNA and RNA, and both molecules exhibit minor grooves ...

This study established a model for the FOT measurement system and analyzed the time-frequency domain response using Fourier analysis. Based on clinical studies of the FOT excitation signal, we first ...