A new, streamlined AAV purification process that is both universal and scalable could ease commercialization constraint.
"The Church gives the name Purgatory to this final purification of the elect, which is entirely different from the punishment of the damned. [Cf. Council of Florence (1439): DS 1304; Council of ...
chosen in this study were commonly used affinity tags in protein purification and detection. In order to take full advantage of the selected aptamers, we next asked whether the selected aptamers could ...
For the injection of retrograde tracer, CTB647 (0.35% w/v in saline; Life Technologies, New Zealand) male Fezf2-Gfp mice (P23–25) were anesthetized by a sub-cutaneous injection of 0.05 mg/kg atropine ...
The purification system is the most intense of the bottles tested. It can filter out chemicals, pesticides, particles, heavy metals, bacteria and viruses. It also takes little to no prep or ...
In summary, the tetracysteine-biarsenical system affords a powerful alternative to GFP tagging for protein visualization. The hexapeptide tag is a minimal structural perturbation relative to ...
Purgatory is not a second-chance for damned souls to repent. Instead it is a state of cleansing and purification for souls destined for heaven. Also Purgatory is not a means to earn our way to ...
1) and cloned it into AAV-MAG-mcherry. The sequences of jGCaMP7f gene and jGCaMP7s were amplified by PCR from pGP- AAV-syn-jGCaMP7f-WPRE and pGP-AAV-syn-jGCaMP7s-WPRE (a gift from Douglas Kim & GENIE ...
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This valuable study presents a mouse gastruloid model that can be used to generate hematopoietic progenitors as well as leukemic cells. However, in its current form, the manuscript is inadequate ...