1/ 🧵 Tackling Ambient RNA in Single-Cell RNA-seq Data Ambient RNA contamination can skew single-cell RNA sequencing (scRNA-seq) results. Let's explore its impact and solutions. 2/ What is ...

RNA sequencing refers to techniques used to determine the sequence of RNA molecules. It includes high-throughput shotgun sequencing of cDNA molecules obtained by reverse transcription from RNA ...

RNA (ribonucleic acid) is a nucleic acid polymer where the carbohydrate is ribose. RNA is generally single-stranded, as DNA is transcribed by RNA polymerases into mRNA (messenger RNA), which is ...

State Key Laboratory of Virology and Biosafety, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan 430071, China University of Chinese Academy of ...

The cells were partitioned into Gel Bead-In-Emulsions and barcoded cDNA libraries ... Thus, we applied a slightly different criteria to remove supposed ambient RNA contamination (<1,700 UMI for ...

You’ve certainly heard about antibodies, peptides, and even DNA as therapeutics, but what about RNA? In this review, we take a look at what’s happening in a field that some believe may be as big a ...

SEVEN patients, two of whom were critically ill, have been infected with a contaminated gel during NHS procedures. The patients across four regions in the UK were found to be infected with ...

To interrogate subclonal heterogeneity within tumor populations in pediatric EPN, we performed single-cell RNA-seq on EPN samples across PF-A and ST regions ... Germany) was added to remove blood ...

To validate these novel homodimers, we synthesized extended RRE RNA fragments with two dimer sites using in vitro transcription and confirmed their ability to form a dimeric conformation through ...

Dec. 11, 2024 at 4:48 p.m. ET on TipRanks.com ...