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For the migration assay, cells were suspended in serum-free medium (Gibco, Cat. No. 31603-029) at a density of 1×10^5 cells/mL and added to the upper chamber of a Transwell insert (Corning, Cat. No.

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Images of the wound were captured after 24 hours of incubation. The wound area was measured using Image J. 24-well transwell chambers, coated with or without Matrigel (Corning, NY, USA, 354480, 3422), ...