1| Incubate the cell lysate with 50 μl of a 50% slurry of glutathione-agarose beads and 25 μg of GST for 2 h at 4 °C with end-over-end mixing. The amount of lysate needed to detect an ...

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To address the roles of these interactions, we utilized single-molecule fluorescence assays to monitor Cse4 during kinetochore assembly. We found that Okp1/Ame1 and Scm3 independently stabilize Cse4 ...

Data presented in this useful report suggest a potentially new model for chemotaxis regulation in the gram-negative bacterium P. putida. Data supporting interactions between CheA and the ...

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When you buy through our links, Business Insider may earn an affiliate commission. Learn more Dogs who pull on leash can be very difficult to walk. However, one of the best no-pull dog harnesses ...

CHARLOTTE, N.C. — The Clippers are perfecting their finishing kick, and they needed it to secure another road win Friday night. Norman Powell scored 27 points, Kawhi Leonard added 21 points and ...

PDV1 was thought to work corporately with PDV2 to recruit ARC5 to the chloroplast division site in the cytosol. However, we found that the major role of PDV1 is to interact with and regulate the ...

Objective Antimicrobial peptides (AMPs) play essential roles in maintaining gut health and are associated with IBD. This study is to elucidate the effect of angiogenin (ANG), an intestine-secreted AMP ...